OneWorld Biotech’s ProteoWay™ Protein Production Optimization ensures that each stage of the protein expression process — from vector design, host selection, culture conditions and purification to protein characterization — delivers the highest quality biologically active protein possible.
Welcome to OneWorld Biotech, Inc. an R&D Partner Organization! We specialize in handling difficult-to-express proteins and complex expression and purification projects and provide optimal solutions. We offer complete protein production services including gene synthesis and cloning, protein expression and purification, protein characterization, and assay development. Our molecular biology services are tailored to meet client-specific requirements in molecular cloning, RNA synthesis, and DNA/RNA purification.
High levels of protein expression in the host of choice are essential for economical purification. Recombinant protein production is a difficult process and involves evaluation of variety of technologies and careful experimental planning. Because each protein production project is unique, it is essential to select the optimal host expression system. Utilizing the skills and technologies we have mastered over the years, we will help you reach your goals efficiently and cost-effectively. Discover the difference – call 215-942-4004 or email today!
OneWorld Biotech is now pleased to offer expanded and cost-effective NucleoWay™ Molecular Cloning services to the wider scientific community. We have been helping our clients with gene cloning, RNA and mRNA synthesis, plasmid DNA and RNA purification, and other DNA/RNA analysis projects over the past nine years.
Our in-house protein purification process and techniques are customized to a wide variety of expression hosts from small-scale to large-scale production of recombinant proteins. For each protein, we evaluate a combination of purification methods for simplicity and cost to obtain highly pure and biologically active protein.
Mammalian cell biotechnology is a demanding system for the production of recombinant proteins compared to microbial systems. We employ recent developments – genetic, biochemical, metabolic control of cells, glycosylation, bioreactors properties, and new chromatography solutions – to reduce the time and cost required to establish transient and stable protein production.
Our experience with a variety of expression systems enables us to make the right microbial host choice that meets your needs. We are experts in optimizing protein expression using established and new developments in tunable expression, solubility technology, and protein targeting to maximize protein yield, biological activity and purity.
We offer three types of protein expression in insect cells: 1) stable transformation of insect cells for long term continuous production of difficult-to-express cytoplasmic, membrane, nuclear, and secretary proteins, 2) plasmid-based transient transfection for quick purification of protein variants, and 3) Baculovirus expression vector system with transfer vectors for multi-host expression.
Here we wish to give you general procedures for gene synthesis and vector construction, purification tags, fusion proteins, protein conjugates, protein expression, protein purification, protein characterization, and assay development. We assembled these resources to help our clients make the right choices.
OneWorld Biotech celebrates the successful completion of over 8 years of quality- and customer-focused CRO service to biotech and pharmaceutical companies, NIH and academic labs. Thank you so much for your continued support, trust in our services, and for giving us the opportunity to work on exciting projects.