OneWorld Biotech’s ProteoWay™ Protein Production Optimization ensures that each stage of the protein expression process — from vector design, host selection, culture conditions and purification to protein characterization — is optimized to deliver the highest quality biologically active protein possible.
Welcome to OneWorld Biotech, an R&D Partner Organization! We specialize in handling difficult-to-express proteins and complex expression and purification projects and provide optimal solutions. We offer complete protein production services including gene synthesis and cloning, protein expression and purification, protein characterization, and assay development.
High levels of protein expression in the host of choice are essential for economical purification. Recombinant protein production is a difficult process and involves evaluation of variety of technologies and careful experimental planning. With so many choices available, it is essential to select the optimal host expression system and methods to achieve the desired performance and product specifications. Each protein expression and purification project is unique. We will help you, utilizing the skills and technologies we have mastered over the years, reach your goals efficiently and cost-effectively. Discover the difference – call 215-942-4004 or Email today!
Mammalian cell biotechnology constitutes a demanding system for the production of recombinant proteins compared to microbial systems. We employ recent developments – genetic, biochemical, metabolic control of cells, glycosylation, bioreactors properties, and new chromatography solutions – to reduce the time and cost required to establish transient and stable eukaryotic cell-based protein production.
Our experience with a variety of expression systems and our know-how enable us to make the right microbial host choice that meets your needs. We are experts optimizing protein expression using established and new developments in tunable expression, solubility technology, and protein targeting to maximize protein yield, biological activity and purity.
We offer three types of protein expression in insect cells: 1) stable transformation of insect cells for long term continuous production of difficult-to-express cytoplasmic, membrane, nuclear, and secretary proteins, 2) plasmid-based transient transfection for quick purification of protein variants, and 3) Baculovirus expression vector system with transfer vectors for multi-host expression in E. coli, insect, and mammalian cells.
Our in-house protein purification process and techniques are customized to a wide variety of expression hosts from small-scale to large-scale production of recombinant proteins. For each protein, we evaluate a combination of purification methods for simplicity and best economics so that highly pure protein is obtained to meet client needs.
OneWorld Biotech offers a proven scientific and systematic approach to successful custom assay design and manufacture. We use custom-designed reagents (proteins, antibodies, conjugated antibodies, and cell lines) and can also source assay components from third party vendors. OneWorld Biotech has the capability to create a variety of validated assay systems.
OneWorld Biotech, Inc. is now pleased to offer expanded DNA and RNA services to the wider scientific community. We have been helping biotech and pharma clients with custom gene cloning and plasmid construction, DNA purification, RNA and mRNA synthesis, RNA purification, and DNA/RNA analysis projects over the past eight years. DNA/RNA services are an extension of our protein expression services.
To assist in our clients’ in-house R&D efforts we offer unique and special products that are cost-effective and easy-to-use: Purified Proteins, ELISA and other Assay Reagents and Standards, and Tools and Reagents for Expression Vector Construction, Protein Expression, Protein Purification, and Assay Development.
No project is too small, too large, too simple, or too complex for OneWorld Biotech and we are looking forward to accelerating your success and achieving your research goals. We offer an initial free, no obligation consultation session to advise on appropriate procedures and strategies for your project.
Here we wish to give you general procedures for Gene Synthesis and Cloning, Expression Vector Construction, Purification Tags, Fusion Proteins and Protein Conjugates, Protein Expression, Protein Purification, Protein Characterization, and Assay Development. We assembled these resources to help our clients make the right choices and for others interested in learning about protein production. Please contact us for additional information.
OneWorld Biotech celebrates successful completion of over 8 years of quality- and customer-focused CRO service to biotech and pharmaceutical companies and NIH and academic labs. Thank you so much for your continued support and trust in our services and also for giving us the opportunity to work on exciting projects. We are committed to establish productive, long-term, strategic relationship with our clients to accelerate product development and look forward to hearing about your next project. Thank you again.